goat anti pink1  (St Johns Laboratory)

Journal: Journal of neurochemistry

Article Title: Regulation of PINK1 by NR2B-containing NMDA receptors in ischemic neuronal injury.

doi: 10.1111/j.1471-4159.2009.06398.x

Figure Lengend Snippet: Fig. 1 OGD reduces PINK1 protein expression. (a and b) Protein levels of PINK1 were analyzed by western blotting. Each bar graph represents densitometry analysis from six independent experiments. The expression of PINK1 was normalized to that of OGD-untreated control at 0.5 h in (a) and that of OGD-untreated control of 24 h recovery in (b) respectively. (a) Rat cortical neurons were exposed to OGD for 0.5, 1 or 2 h and then recovered for 24 h. The cultures treated with 2 h OGD have significant reduction of PINK1 expression (Student’s t-test, *p < 0.05 vs. 2 h control). (b) Cortical neurons were exposed to OGD for 2 h and then recovered for 24, 48 or 72 h. Reoxygenation causes time-dependent reduction of PINK1 expression

Article Snippet: The goat IgG anti-PINK1 antibody (sc-32584) was purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Journal of neurochemistry

Article Title: Regulation of PINK1 by NR2B-containing NMDA receptors in ischemic neuronal injury.

doi: 10.1111/j.1471-4159.2009.06398.x

Figure Lengend Snippet: Fig. 2 NMDAR antagonists block OGD-induced PINK1 reduction. The cells were exposed to OGD for 2 h and then recovered for 24 h in the presence of 50 lM D-APV, 5 lM MK801 or vehicles respectively. (a) Expression levels of PINK1 were analyzed by western blotting. (b) Bar graph represents densitometry analysis from six independent experi- ments. The expression of PINK1 was normalized to the OGD-un- treated control incubated with vehicles, respectively, in each individual experiment (ANOVA test, *p < 0.05 vs. OGD-untreated cells incubated with vehicles; #p < 0.05 vs. OGD-treated cells incubated with vehi- cles).

Article Snippet: The goat IgG anti-PINK1 antibody (sc-32584) was purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

Techniques: Blocking Assay, Expressing, Western Blot, Control, Incubation

Journal: Journal of neurochemistry

Article Title: Regulation of PINK1 by NR2B-containing NMDA receptors in ischemic neuronal injury.

doi: 10.1111/j.1471-4159.2009.06398.x

Figure Lengend Snippet: Fig. 5 Suppressing protein expression of PINK1 leads to decreased Akt phosphorylation. Cortical neurons were transfected with specific PINK1 siRNA or scrambled control siRNA (SsiRNA) for 48 h. The cells were processed for immunocytochemical staining with PINK1 or pAkt antibody. Arrowheads indicate successfully transfected neurons with GFP expression. (a) PINK1 siRNA reduces the protein expression of PINK1. Bar graph represents quantification of fluorescent intensity of

Article Snippet: The goat IgG anti-PINK1 antibody (sc-32584) was purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

Techniques: Expressing, Phospho-proteomics, Transfection, Control, Staining

Journal: Journal of neurochemistry

Article Title: Regulation of PINK1 by NR2B-containing NMDA receptors in ischemic neuronal injury.

doi: 10.1111/j.1471-4159.2009.06398.x

Figure Lengend Snippet: Fig. 6 Over-expression of PINK1 reduces OGD-induced decrease of Akt phosphorylation. Representative images in the top row panel show that PINK1 cDNA transfection, as labeled by GFP, increases PINK1 expression in cultured cortical neurons, and the panel in the second row shows that the over-expression of PINK1 reduces OGD-induced decrease of PINK1 protein (ANOVA t-test, *p < 0.05 vs. GFP cells without OGD treatment; #p < 0.05 vs. GFP cells treated with OGD;

Article Snippet: The goat IgG anti-PINK1 antibody (sc-32584) was purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

Techniques: Over Expression, Phospho-proteomics, Transfection, Labeling, Expressing, Cell Culture

Journal: Journal of neurochemistry

Article Title: Regulation of PINK1 by NR2B-containing NMDA receptors in ischemic neuronal injury.

doi: 10.1111/j.1471-4159.2009.06398.x

Figure Lengend Snippet: Fig. 7 PINK1 over-expression attenuates OGD-induced neuronal death. Representa- tive images show that PINK1 over-expres- sion reduces OGD-induced neuronal death. Summarized data indicate that enhancing PINK1 expression protects against OGD- induced neuronal death (ANOVA test, *p < 0.05 vs. GFP cells without OGD treat- ment; #p < 0.05 vs. GFP cells treated with OGD; n = 65 for each group). Neuronal death assays were performed from six independent dissections (cells were coun- ted in one petri dish per dissection).

Article Snippet: The goat IgG anti-PINK1 antibody (sc-32584) was purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

Techniques: Over Expression, Expressing, Dissection

Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.

doi: 10.1038/jcbfm.2008.167

Figure Lengend Snippet: Figure 1 Representative Western blot for DJ-1, PINK1, and a-Syn. Transient ischemia affects expression profile of DJ-1, PINK1, and a-Syn at 8 h of reperfusion. 20 mg of protein from each samples were run on the gel for 90 mins at 20 mA. The proteins on the gel were then transferred to a PVDF membrane, they were then incubated with primary antibodies at 1:1000 dilution for 20 h at 41C. The blots were developed using Chromogenic detection method.

Article Snippet: The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).

Techniques: Western Blot, Expressing, Membrane, Incubation

Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.

doi: 10.1038/jcbfm.2008.167

Figure Lengend Snippet: Figure 2 Immunostaining against DJ-1, PINK1, and a-Syn in motor neurons in a sham spinal cord (A, D, and G); at 8 h (B, E, and H), 1 day (C, F, and I) of reperfusion. Spinal cord sections were incubated with primary antibodies for 20 h at 41C, respectively. The primary antibodies (1:200 dilution, respectively) used were the same as those used for Western blot analysis noted above. The slices were colorized with DAB/H2O2 solution. Bar = 100 mm.

Article Snippet: The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).

Techniques: Immunostaining, Incubation, Western Blot

Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.

doi: 10.1038/jcbfm.2008.167

Figure Lengend Snippet: Figure 3 Co-localization of DJ-1 and PINK1 (A–C), a-Syn and PINK1 in motor neurons (D–F) at 8 h after ischemia. Then, the sections were incubated with DJ-1 antibodies 1:100 simultaneously with PINK1, and the sections were incubated with a-Syn antibodies 1:100 simultaneously with PINK1. The sections were detected by using donkey anti-mouse lgG linked with fluorescein isothiocyanate 1:50 and donkey anti-goat lgG linked with Texas Red I:50 and observed using fluorescein microscopy. Bar = 50 mm.

Article Snippet: The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).

Techniques: Incubation, Microscopy